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Bio::Tools::Primer::Feature - position of a single primer

Appendix

       The rest of the documentation details each of the object methods.  Internal methods are usually preceded
       with a _

perl v5.32.1                                       2021-08-15                   Bio::Tools::Primer::Feature(3pm)

Author - Ewan Birney

       Email birney-at-ebi.ac.uk

Description

       Primer Features represents one primer in a primer pair. This object is mainly for designing primers, and
       probably principly used in the primer design system

Feedback

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       Please direct usage questions or support issues to the mailing list:

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Name

       Bio::Tools::Primer::Feature - position of a single primer

Synopsis

           use Bio::Tools::Primer::Feature;

           my $pf = Bio::Tools::Primer::Feature->new( -start => $start, -end => $end, -strand => $strand);
           $pf->attach_seq($seq);

           # is a SeqFeatureI

           print "primer starts at ",$pf->start," with sequence ",$pf->seq->seq(),"\n";

           # helper functions

           print "GC percentage ",$pf->gc(),"\n";
           print "has inversion of size 4 at ",$pf->inversion(4),"\n";

See Also