Bio::Graphics::Browser2::DataLoader::useq - data loader for the USeq archive
Contents
Description
A data loader for the USeq archive, recognized by the file extension ".useq". See
<http://useq.sourceforge.net/useqArchiveFormat.html> for information regarding the file format. Briefly,
this format can store either genomic intervals with or without text and/or scores, or quantitative scores
along a chromosome (point data).
There is currently no native BioPerl adaptor for the USeq archive. Upon upload, the file is converted to
either a UCSC BigBed or BigWig format, depending upon the file contents. Stranded point data may be
converted into two BigWig files, each for the Plus and Minus strand. Configuration files are generated as
appropriate for the converted files.
Name
Bio::Graphics::Browser2::DataLoader::useq - data loader for the USeq archive
Setup
To process the USeq archive, the USeq package (<http://useq.sourceforge.net> must be installed in a
globally accessible path. This location is searched upon initiation. Common paths to search include
"/usr", "/usr/local", "/opt", "/opt/gbrowse", "/data", "/data/opt", and "/Applications", in that order.
The USeq App "USeq2UCSCBig" (a jar file) is used to convert the USeq archive. This app requires three
binary executables: "java" and the two UCSC utilities "bedToBigBed" and "wigToBigWig". These are searched
for in the environment $PATH variable. The UCSC utilities are available at
<http://hgdownload.cse.ucsc.edu/admin/exe/>. The USeq Apps requires Java 1.6+.
Failure to find the paths for all three will result in failure to process the .useq file.
