lambda_indexer - indexer for creating lambda-compatible databases
Contents
Description
Lambda is a local aligner optimized for many query sequences and searches in protein space. It is
compatible to BLAST, but much faster than BLAST and many other comparable tools.
Detailed information is available in the wiki: <https://github.com/seqan/lambda/wiki>
This is the indexer_binary for creating lambda-compatible databases.
Legal
lambda_indexerCopyright: 2013-2017 Hannes Hauswedell, released under the GNU GPL v3 (or later);
2016-2017 Knut Reinert and Freie Universität Berlin, released under the 3-clause-BSDL
SeqAnCopyright: 2006-2015 Knut Reinert, FU-Berlin; released under the 3-clause BSDL.
Inyouracademicworkspleasecite: Hauswedell et al (2014); doi: 10.1093/bioinformatics/btu439
For full copyright and/or warranty information see --copyright.
lambda_indexer 1.0.2 Feb 8 2020 LAMBDA_INDEXER(1)
Name
lambda_indexer - indexer for creating lambda-compatible databases
Options
-h, --help
Display the help message.
-hh, --full-help
Display the help message with advanced options.
--version-checkBOOL
Turn this option off to disable version update notifications of the application. One of 1, ON,
TRUE, T, YES, 0, OFF, FALSE, F, and NO. Default: 1.
--version
Display version information.
--copyright
Display long copyright information.
-v, --verbosityINTEGER
Display more/less diagnostic output during operation: 0 [only errors]; 1 [default]; 2 [+run-time,
options and statistics]. In range [0..2]. Default: 1.
InputOptions:-d, --databaseINPUT_FILE
Database sequences. Valid filetypes are: .sam[.*], .raw[.*], .gbk[.*], .frn[.*], .fq[.*],
.fna[.*], .ffn[.*], .fastq[.*], .fasta[.*], .faa[.*], .fa[.*], .embl[.*], and .bam, where * is any
of the following extensions: gz, bz2, and bgzf for transparent (de)compression.
-s, --segfileINPUT_FILE
SEG intervals for database(optional). Valid filetype is: .seg.
OutputOptions:-di, --db-index-typeSTRING
Suffix array or full-text minute space. One of sa and fm. Default: fm.
--truncate-idsSTRING
Truncate IDs at first whitespace. This saves a lot of space and is irrelevant for all LAMBDA
output formats other than BLAST Pairwise (.m0). One of on and off. Default: on.
AlphabetsandTranslation:-p, --programSTRING
Blast Operation Mode. One of blastn, blastp, blastx, tblastn, and tblastx. Default: blastx.
-g, --genetic-codeINTEGER
The translation table to use (not for BlastN, BlastP). See
https://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi?mode=c for ids (default is generic).
Default: 1.
-ar, --alphabet-reductionSTRING
Alphabet Reduction for seeding phase (ignored for BLASTN). One of none and murphy10. Default:
murphy10.
Algorithm:-a, --algorithmSTRING
Algorithm for SA construction (also used for FM; see Memory Requirements below!). One of
mergesort, quicksortbuckets, quicksort, radixsort, and skew7ext. Default: radixsort.
-t, --threadsINTEGER
number of threads to run concurrently (ignored if a == skew7ext).
-td, --tmp-dirSTRING
temporary directory used by skew, defaults to working directory.
Remarks
Please see the wiki (<https://github.com/seqan/lambda/wiki>) for more information on which indexes to
chose and which algorithms to pick.
Note that the indexes created are binary and not compatible between different CPU endiannesses. Also the
on-disk format is still subject to change between Lambda versions.
Synopsis
lambda_indexer [OPTIONS] -d DATABASE.fasta
