primers - Simple version of primer3 to pick PCR primers

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EMBOSS 6.4.0                                       05/11/2012                                        PRIMERS(1e)

primers is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It
       is part of the "Nucleic:Primers" command group(s).

       primers - Simple version of primer3 to pick PCR primers

Inputsection-sequenceseqall
           The sequence from which to choose primers. The sequence must be presented 5' -> 3'

   Additionalsection-targetregionrange
           If one or more Targets is specified then a legal primer pair must flank at least one of them. A
           Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair
           polymorphism. The value should be a space-separated list of <start>,<end> pairs where <start> is the
           index of the first base of a Target, and <end> is the last E.g. 50,51 requires primers to surround
           the 2 bases at positions 50 and 51.

       -minsizeinteger
           Minimum acceptable length of a primer. Must be greater than 0 and less than or equal to MAX-SIZE.
           Default value: 18

       -maxsizeinteger
           Maximum acceptable length (in bases) of a primer. Currently this parameter cannot be larger than 35.
           This limit is governed by the maximum oligo size for which Primer3's melting-temperature is valid.
           Default value: 27

       -maxdifftmfloat
           Maximum acceptable (unsigned) difference between the melting temperatures of the forward and reverse
           primers. Default value: 100.0

   Outputsection-explainflagboolean
           If this flag is non-0, produce LEFT-EXPLAIN and RIGHT-EXPLAIN, output, which are intended to provide
           information on the number of primer pairs that Primer3 examined, and statistics on the number
           discarded for various reasons. Default value: N

       -numreturninteger
           The maximum number of primer pairs to return. Primer pairs returned are sorted by their 'quality', in
           other words by the value of the objective function (where a lower number indicates a better primer
           pair). Caution: setting this parameter to a large value will increase running time. Default value: 5

       -outfileoutfile

       primers is fully documented via the tfm(1) system.

primers-sequenceseqall [-targetregionrange] [-minsizeinteger] [-maxsizeinteger] [-maxdifftmfloat]
               [-explainflagboolean] [-numreturninteger] -outfileoutfileprimers-help