USAGE:
Sibelia
{-s <loose|fine|far>|-k <file name>} [-o <dir name>] [--noblocks] [-r] [-a] [-m <integer>] [-q]
[-g] [-v] [-t <dir name>] [--lastk <integer > 1>] [--allstages] [--gff] [--nopostprocess]
[--correctboundaries] [-i <integer > 0>] [--] [--version] [-h] <fasta files with genomes> ...
Where:
-s <loose|fine|far>, --parameters <loose|fine|far>
(OR required)
Parameters set, used for the simplification. Option
"loose" produces fewer blocks, but they are larger ("fine" is opposite).
-- OR ---k <file name>, --stagefile <file name>
(OR required)
File that contains manually chosen simplifications
parameters. See USAGE file for more information.
-o <dir name>, --outdir <dir name>
Directory where output files are written
--noblocks
Do not compute synteny blocks
-r, --inram
Perform all computations in RAM, don't create temp files.
-a, --sharedonly
Output only blocks that occur exactly once in each input sequence.
-m <integer>, --minblocksize <integer>
Minimum size of a synteny block, default value = 5000 BP.
-q, --sequencesfile
Output sequences of synteny blocks (FASTA format).
-g, --graphfile
Output resulting condensed de Bruijn graph (in dot format).
-v, --visualize
Draw circos diagram with blocks at different stages.
-t <dir name>, --tempdir <dir name>
Directory where temporary files are stored.
--lastk <integer > 1>
Value of K used for the synteny blocks inferring.
--allstages
Output coordinates of synteny blocks from all stages
--gff
Use GFF format for reporting blocks coordinates
--nopostprocess
Do not perform postprocessing (stripe gluing).
--correctboundaries
Correct boundaries of unique synteny blocks.
-i <integer > 0>, --maxiterations <integer > 0>
Maximum number of iterations during a stage of simplification, default = 4.
--, --ignore_rest
Ignores the rest of the labeled arguments following this flag.
--version
Displays version information and exits.
-h, --help
Displays usage information and exits.
<fasta files with genomes>
(accepted multiple times)
(required)
FASTA file(s) with nucleotide sequences.
Program for finding syteny blocks in closely related genomes