Inputsection-sequencesequenceAdditionalsection-tablelist
Genetics code used for the translation
-firstorfboolean
Count the beginning of a sequence as a possible ORF, even if it's inferior to the minimal ORF size.
Default value: Y
-lastorfboolean
Count the end of a sequence as a possible ORF, even if it's not finishing with a STOP, or inferior to
the minimal ORF size. Default value: Y
-mstartboolean
Displays only ORFs starting with an M. Default value: N
Outputsection-outfileoutfile-outseqseqoutall
ORF sequence output
-reverseboolean
Display also the translation of the DNA sequence in the 3 reverse frames Default value: Y
-orfminsizeinteger
Minimum size of Open Reading Frames (ORFs) to display in the translations. Default value: 1
-uppercaserange
Regions to put in uppercase. If this is left blank, then the sequence case is left alone. A set of
regions is specified by a set of pairs of positions. The positions are integers. They are separated
by any non-digit, non-alpha character. Examples of region specifications are: 24-45, 56-78 1:45,
67=99;765..888 1,5,8,10,23,45,57,99
-highlightrange
Regions to colour if formatting for HTML. If this is left blank, then the sequence is left alone. A
set of regions is specified by a set of pairs of positions. The positions are integers. They are
followed by any valid HTML font colour. Examples of region specifications are: 24-45 blue 56-78
orange 1-100 green 120-156 red A file of ranges to colour (one range per line) can be specified as
'@filename'.
-numberboolean
Number the sequence at the beginning and the end of each line. Default value: Y
-widthinteger
Number of nucleotides displayed on each line Default value: 60
-lengthinteger-margininteger
Default value: 10
-nameboolean
Set this to be false if you do not wish to display the ID name of the sequence. Default value: Y
-descriptionboolean
Set this to be false if you do not wish to display the description of the sequence. Default value: Y
-offsetinteger
Number from which you want the DNA sequence to be numbered. Default value: 1
-htmlboolean
Default value: N