-h, --help
show this help message and exit
--sam SAM
Input SAM file of alignments (if this file doesn't exist, the alignment will be performed with
results saved to this file - you can use the aligner arguments with this script)
--ref REF
FASTA file containing one or more reference sequences
--reads READS
FASTQ file of long reads
--min_len MIN_LEN
Minimum alignment length (bp) - exclude alignments shorter than this length (default: 100)
--error_window_size ERROR_WINDOW_SIZE
Window size for error summaries (default: 100)
--depth_window_size DEPTH_WINDOW_SIZE
Window size for depth summaries (default: 100)
--error_rate_threshold ERROR_RATE_THRESHOLD
Threshold for high error rates, expressed as the fraction between the mean error rate and the
random alignment error rate (default: 0.3)
--depth_p_val DEPTH_P_VAL
P-value for low/high depth thresholds (default: 0.001)
--window_tables WINDOW_TABLES
Path and/or prefix for table files summarising reference errors for reference windows (default: do
not save window tables)
--base_tables BASE_TABLES
Path and/or prefix for table files summarising reference errors at each base (default: do not save
base tables)
--html HTML
Path for HTML report (default: do not save HTML report)
--threads THREADS
Number of CPU threads used to align (default: the number of available CPUs)
--verbosity VERBOSITY
Level of stdout information (0 to 2) (default: 1)