Number of arguments is not correct!
sorted_alignment_file : Can be either in SAM/BAM/CRAM format, must be sorted wig_output :
Output wiggle file's name, e.g. output.wig wiggle_name : the name of this wiggle plot
--no-fractional-weight : If this is set, RSEM will not look for "ZW" tag and each alignment appeared in
the BAM file has weight 1. Set this if your BAM file is not generated by RSEM. Please note that this
option must be at the end of the command line.
rsem-bam2wig 1.3.3+dfsg April 2024 RSEM-BAM2WIG(1)