usage: average_nucleotide_identity.py [-h] [--version] -o OUTDIRNAME -i
INDIRNAME [-v] [-f] [-s FRAGSIZE]
[-l LOGFILE] [--skip_nucmer] [--skip_blastn] [--noclobber] [--nocompress] [-g] [--gformat GFORMAT]
[--gmethod {mpl,seaborn}] [--labels LABELS] [--classes CLASSES] [-m {ANIm,ANIb,ANIblastall,TETRA}]
[--scheduler {multiprocessing,SGE}] [--workers WORKERS] [--SGEgroupsize SGEGROUPSIZE] [--SGEargs
SGEARGS] [--maxmatch] [--nucmer_exe NUCMER_EXE] [--filter_exe FILTER_EXE] [--blastn_exe
BLASTN_EXE] [--makeblastdb_exe MAKEBLASTDB_EXE] [--blastall_exe BLASTALL_EXE] [--formatdb_exe
FORMATDB_EXE] [--write_excel] [--rerender] [--subsample SUBSAMPLE] [--seed SEED] [--jobprefix
JOBPREFIX]
optionalarguments:-h, --help
show this help message and exit
--version
show program's version number and exit
-o OUTDIRNAME, --outdir OUTDIRNAME
Output directory (required)
-i INDIRNAME, --indir INDIRNAME
Input directory name (required)
-v, --verbose
Give verbose output
-f, --force
Force file overwriting
-s FRAGSIZE, --fragsize FRAGSIZE
Sequence fragment size for ANIb (default 1020)
-l LOGFILE, --logfile LOGFILE
Logfile location
--skip_nucmer
Skip NUCmer runs, for testing (e.g. if output already present)
--skip_blastn
Skip BLASTN runs, for testing (e.g. if output already present)
--noclobber
Don't nuke existing files
--nocompress
Don't compress/delete the comparison output
-g, --graphics
Generate heatmap of ANI
--gformat GFORMAT
Graphics output format(s) [pdf|png|jpg|svg] (default pdf,png,eps meaning three file formats)
--gmethod {mpl,seaborn}
Graphics output method (default mpl)
--labels LABELS
Path to file containing sequence labels
--classes CLASSES
Path to file containing sequence classes
-m {ANIm,ANIb,ANIblastall,TETRA}, --method {ANIm,ANIb,ANIblastall,TETRA}
ANI method (default ANIm)
--scheduler {multiprocessing,SGE}
Job scheduler (default multiprocessing, i.e. locally)
--workers WORKERS
Number of worker processes for multiprocessing (default zero, meaning use all available cores)
--SGEgroupsize SGEGROUPSIZE
Number of jobs to place in an SGE array group (default 10000)
--SGEargs SGEARGS
Additional arguments for qsub
--maxmatch
Override MUMmer to allow all NUCmer matches
--nucmer_exe NUCMER_EXE
Path to NUCmer executable
--filter_exe FILTER_EXE
Path to delta-filter executable
--blastn_exe BLASTN_EXE
Path to BLASTN+ executable
--makeblastdb_exe MAKEBLASTDB_EXE
Path to BLAST+ makeblastdb executable
--blastall_exe BLASTALL_EXE
Path to BLASTALL executable
--formatdb_exe FORMATDB_EXE
Path to BLAST formatdb executable
--write_excel
Write Excel format output tables
--rerender
Rerender graphics output without recalculation
--subsample SUBSAMPLE
Subsample a percentage [0-1] or specific number (1-n) of input sequences
--seed SEED
Set random seed for reproducible subsampling.
--jobprefix JOBPREFIX
Prefix for SGE jobs (default ANI).