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Barcode_split - simple tool to split sequencing summary report in per barcodes

Author

        This manpage was written by Nilesh Patra for the Debian distribution and
        can be used for any other usage of the program.

Barcode_split 2.5.2                               December 2020                                 BARCODE_SPLIT(1)

Description

       usage: Barcode_split [-h] [--version] --summary_file [SUMMARY_FILE ...]

       [--barcode_file [BARCODE_FILE ...]]
              [--output_dir  OUTPUT_DIR] [--output_unclassified] [--min_barcode_percent MIN_BARCODE_PERCENT] [-v
              | -q]

       Barcode_split is a simple tool to split sequencing summary report in per barcodes

   optionalarguments:-h, --help
              show this help message and exit

       --version
              show program's version number and exit

       --summary_file [SUMMARY_FILE ...], -f [SUMMARY_FILE ...]
              Path to a sequencing_summary generated by Albacore  1.0.0  +  (read_fast5_basecaller.py)  /  Guppy
              2.1.3+  (guppy_basecaller).  One can also pass multiple space separated file paths or a UNIX style
              regex matching multiple files

       --barcode_file [BARCODE_FILE ...], -b [BARCODE_FILE ...]
              Path to the barcode_file generated by Guppy 2.1.3+ (guppy_barcoder) or Deepbinner 0.2.0+. One  can
              also pass multiple space separated file paths or a UNIX style regex matching multiple files

       --output_dir OUTPUT_DIR, -o OUTPUT_DIR
              Folder where to output split barcode data (default: current dir

       --output_unclassified, -u
              If given, unclassified barcodes are also written in a file. By default they are skiped

       --min_barcode_percent MIN_BARCODE_PERCENT, -p MIN_BARCODE_PERCENT
              Minimal  percent  of  total  reads  to retain barcode label. If below, the barcode value is set as
              `unclassified` (default: 0.1)

       -v, --verbose
              Increase verbosity

       -q, --quiet
              Reduce verbosity

Name

       Barcode_split - simple tool to split sequencing summary report in per barcodes

See Also