digest - Reports on protein proteolytic enzyme or reagent cleavage sites
Contents
Bugs
Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to
the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).
Copyright
This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be
redistributed under the same terms as EMBOSS itself.
EMBOSS 6.4.0 05/11/2012 DIGEST(1e)
Description
digest is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It
is part of the "Protein:Motifs" command group(s).
Name
digest - Reports on protein proteolytic enzyme or reagent cleavage sites
Options
Inputsection-seqallseqall-mwdatadatafile
Molecular weight data for amino acids Default value: Emolwt.dat
Requiredsection-menulist
Default value: 1
-monoboolean
Default value: N
Advancedsection-unfavouredboolean
Trypsin will not normally cut after 'KR' if they are followed by any of 'KRIFLP'. Lys-C will not
normally cut after 'K' if it is followed by 'P'. Arg-C will not normally cut after 'R' if it is
followed by 'P'. V8-bicarb will not normally cut after 'E' if it is followed by any of 'KREP'.
V8-phosph will not normally cut after 'DE' if they are followed by 'P'. Chymotrypsin will not
normally cut after 'FYWLM' if they are followed by 'P'. Specifying unfavoured shows these unfavoured
cuts as well as the favoured ones.
-raggingboolean
Allows semi-specific and non-specific digestion. This option is particularly useful for generating
lists of peptide sequences for protein identification using mass-spectrometry.
-terminilist
Default value: 1
Outputsection-overlapboolean
Used for partial digestion. Shows all cuts from favoured cut sites plus 1..3, 2..4, 3..5 etc but not
(e.g.) 2..5. Overlaps are therefore fragments with exactly one potential cut site within it.
-allpartialsboolean
As for overlap but fragments containing more than one potential cut site are included.
-outfilereportSee Also
digest is fully documented via the tfm(1) system.
Synopsis
digest-seqallseqall-mwdatadatafile-menulist-monoboolean-unfavouredboolean-raggingboolean-terminilist-overlapboolean-allpartialsboolean-outfilereportdigest-help
