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estimateExpression - estimate expression given precomputed probabilities of (observed) reads alignments

Description

       Estimates expression given precomputed probabilities of (observed) reads' alignments.

              Uses MCMC sampling algorithm to produce relative abundance or RPKM.

Name

       estimateExpression - estimate expression given precomputed probabilities of (observed) reads alignments

Options

--help

              Show this help information.

       --MCMC_burnIn=<MCMC_burnIn>

              Length of sampler's burn in period. (default: 1000)

       --MCMC_chainsN=<MCMC_chainsN>

              Number of parallel chains used. At least two chains will be used. (default: 4)

       --MCMC_dirAlpha=<MCMC_dirAlpha>

              Alpha parameter for the Dirichlet distribution. (default: 1)

       --MCMC_samplesDOmax

              Produce maximum number of samples (samplesNmax) in second iteration and quit. (default: Off)

       --MCMC_samplesN=<MCMC_samplesN>

              Initial number of samples produced. Doubles after every iteration. (default: 1000)

       --MCMC_samplesNmax=<MCMC_samplesNmax>

              Maximum  number  of samples produced in one iteration. After producing samplesNmax samples sampler
              finishes. (default: 50000)

       --MCMC_samplesSave=<MCMC_samplesSave>

              Number of samples recorder in total. (default: 1000)

       --MCMC_scaleReduction=<MCMC_scaleReduction>

              Target scale reduction, sampler finishes after this value is met. (default: 1.2)

       -G ,   --gibbs

              Use Gibbs sampling instead of collapsed Gibbs sampling. (default: Off)

       -o <outFilePrefix> ,   --outPrefix=<outFilePrefix>

              Prefix for the output files.

       -O <outputType> ,   --outType=<outputType>

              Output type (theta, RPKM, counts, tau). (default: theta)

       -p <parFileName> ,   --parFile=<parFileName>

              File containing parameters for the sampler, which can be otherwise specified by  --MCMC*  options.
              As the file is checked after every MCMC iteration, the parameters can be adjusted while running.

       -P <procN> ,   --procN=<procN>

              Limit the maximum number of threads to be used. (Default is the number of MCMC chains.)

       --scaleReduction

              Use  scale  reduction as stopping criterion, instead of computing effective sample size. (default:
              Off)

       -s <seed> ,   --seed=<seed>

              Random initialization seed.

       --thetaActFile=<thetaActFileName>

              File for logging noise parameter theta^{act}.

       -t <trInfoFileName> ,   --trInfoFile=<trInfoFileName>

              File containing transcript information. (Necessary for RPKM)

       -v ,   --verbose

              Verbose output. (default: Off)

estimateExpression 0.7.5+dfsg                    September 2021                            ESTIMATEEXPRESSION(1)

Synopsis

estimateExpression-o<outFilePrefix>  [OPTIONS] [probfile]

See Also