kmerresistance - correlates mapped genes with the predicted species of WGS samples
Contents
Description
KmerResistance correlates mapped genes with the predicted species of WGS samples, where this this allows
for identification of genes in samples which have been poorly sequenced or high accuracy predictions for
samples with contamination. KmerResistance has one dependency, namely KMA to perform the mapping, which
is also freely available.
Examples
Mapping reads against resistance genes:
kmerresistance -i sample_1.fastq sample_2.fastq -o out -t_db ResFinder -s_db bacteria
Name
kmerresistance - correlates mapped genes with the predicted species of WGS samples
Options
-i Input/query file name REQUIRED
-o Output file REQUIRED
-t_db Template DB REQUIRED
-s_db Species DB REQUIRED
-id ID threshhold 70.0
-dct Depth correction threshhold 0.1
-kma alternative KMA kma
KMAoptions:-i Input/query file name None REQUIRED
-o Output file None REQUIRED
-t_db Template DB None REQUIRED
-k Kmersize 16
-e evalue 0.05
-delta Align in pieces of delta 511
-mem_mode
Use kmers to choose best template, and save memory False
-ex_mode
Searh kmers exhaustively False
-deCon Remove contamination False
-dense Do not allow insertions in assembly False
-ref_fsa
Consensus sequnce will have "n" instead of gaps False
-matrix
Print assembly matrix False
-mp Minimum phred score 30
-5p Cut a constant number of nucleotides from the 5 prime. 0
-Sparse
Run KmerFinder False
-ID Minimum ID 1.0%
-ss Sparse sorting (q,c,d) q
-shm Use shared DB made by kma_shm 0 (lvl)
-swap Swap DB to disk 0 (lvl)
-1t1 Skip HMM False
-boot Bootstrap sequence False
-mrs Minimum alignment score, normalized to alignment length 0.5
-reward
Score for match 1
-penalty
Penalty for mismatch -2-gapopen
Penalty for gap opening -3-gapextend
Penalty for gap extension -1-h Shows this help message
