usage: macs2 [-h] [--version]
{callpeak,bdgpeakcall,bdgbroadcall,bdgcmp,bdgopt,cmbreps,bdgdiff,filterdup,predictd,pileup,randsample,refinepeak}
...
macs2 -- Model-based Analysis for ChIP-Sequencing
positionalarguments:
{callpeak,bdgpeakcall,bdgbroadcall,bdgcmp,bdgopt,cmbreps,bdgdiff,filterdup,predictd,pileup,randsample,refinepeak}
callpeak
Main MACS2 Function: Call peaks from alignment results.
bdgpeakcall
Call peaks from bedGraph output. Note: All regions on the same chromosome in the bedGraph file
should be continuous so only bedGraph files from MACS2 are accpetable.
bdgbroadcall
Call broad peaks from bedGraph output. Note: All regions on the same chromosome in the bedGraph
file should be continuous so only bedGraph files from MACS2 are accpetable.
bdgcmp Deduct noise by comparing two signal tracks in bedGraph. Note: All regions on the same chromosome
in the bedGraph file should be continuous so only bedGraph files from MACS2 are accpetable.
bdgopt Operations on score column of bedGraph file. Note: All regions on the same chromosome in the
bedGraph file should be continuous so only bedGraph files from MACS2 are accpetable.
cmbreps
Combine BEDGraphs of scores from replicates. Note: All regions on the same chromosome in the
bedGraph file should be continuous so only bedGraph files from MACS2 are accpetable.
bdgdiff
Differential peak detection based on paired four bedgraph files. Note: All regions on the same
chromosome in the bedGraph file should be continuous so only bedGraph files from MACS2 are
accpetable.
filterdup
Remove duplicate reads at the same position, then save the rest alignments to BED or BEDPE file.
If you use ' --keep-dup all option', this script can be utilized to convert any acceptable format
into BED or BEDPE format.
predictd
Predict d or fragment size from alignment results. *Will NOT filter duplicates*
pileup Pileup aligned reads with a given extension size (fragment size or d in MACS language). Note there
will be no step for duplicate reads filtering or sequencing depth scaling, so you may need to do
certain pre/postprocessing.
randsample
Randomly sample number/percentage of total reads.
refinepeak
(Experimental) Take raw reads alignment, refine peak summits and give scores measuring balance of
waston/crick tags. Inspired by SPP.
options:-h, --help
show this help message and exit
--version
show program's version number and exit
For command line options of each command, type: macs2 COMMAND -h
macs2 2.2.7.1 April 2022 MACS2(1)