micro_razers - Map small RNA reads possibly containing 3' adapter sequence
Contents
Description
MicroRazerS uses a prefix-based mapping strategy to map small RNA reads possibly containing 3' adapter
sequence.
Input to MicroRazerS is a reference genome file and a file with single-end reads. Use - to read the reads
from stdin.
(c) Copyright 2009 by Anne-Katrin Emde.
Name
micro_razers - Map small RNA reads possibly containing 3' adapter sequence
Options
-h, --help
Display the help message.
--version
Display version information.
MainOptions::-o, --outputOUTPUT_FILE
Change output filename. (use - to dump to stdout in razers format) Default: <READSFILE>.razers.
Valid filetypes are: .sam and .razers.
-rr, --recognition-rateDOUBLE
set the percent recognition rate In range [80..100]. Default: 100.
-sL, --seed-lengthINTEGER
seed length In range [10..inf]. Default: 16.
-sE, --seed-error
allow for one error in the seed
-f, --forward
map reads only to forward strands.
-r, --reverse
map reads only to reverse strands.
-mN, --match-N
'N' matches with all other characters
-m, --max-hitsINTEGER
output only NUM of the best hits In range [1..inf]. Default: 100.
-pa, --purge-ambiguous
purge reads with more than max-hits best matches
-lm, --low-memory
decrease memory usage at the expense of runtime
-v, --verbose
verbose mode
-vv, --vverbose
very verbose mode
OutputOptions::-a, --alignment
dump the alignment for each match
-gn, --genome-namingINTEGER
Select how genomes are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In range [0..1].
Default: 0.
-rn, --read-namingINTEGER
Select how reads are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In range [0..1].
Default: 0.
-so, --sort-orderINTEGER
Select how matches are sorted. 0 = read number, 1 = genome position. In range [0..1]. Default: 0.
-pf, --position-formatINTEGER
Select begin/end position numbering (see Coordinate section below). 0 = gap space, 1 = position
space. In range [0..1]. Default: 0.
micro_razers 1.0.11 [tarball] MICRO_RAZERS(1)
Required Arguments
ARGUMENT0INPUT_FILE
A reference genome file. Valid filetypes are: .sam[.*], .raw[.*], .gbk[.*], .frn[.*], .fq[.*],
.fna[.*], .ffn[.*], .fastq[.*], .fasta[.*], .faa[.*], .fa[.*], .embl[.*], and .bam, where * is any
of the following extensions: gz, bz2, and bgzf for transparent (de)compression.
READS List of INPUT_FILE's
Either one (single-end) or two (paired-end) read files. Valid filetypes are: .sam[.*], .raw[.*],
.gbk[.*], .frn[.*], .fq[.*], .fna[.*], .ffn[.*], .fastq[.*], .fasta[.*], .faa[.*], .fa[.*],
.embl[.*], and .bam, where * is any of the following extensions: gz, bz2, and bgzf for transparent
(de)compression.
Synopsis
micro_razers [OPTIONS] <GENOMEFILE> <READSFILE>
