mosdepth 0.3.10
Usage: mosdepth [options] <prefix> <BAM-or-CRAM>
Arguments:
<prefix>
outputs: `{prefix}.mosdepth.global.dist.txt`
`{prefix}.mosdepth.summary.txt`
`{prefix}.per-base.bed.gz` (unless -n/--no-per-base is specified) `{prefix}.regions.bed.gz` (if
--by is specified) `{prefix}.quantized.bed.gz` (if --quantize is specified)
`{prefix}.thresholds.bed.gz` (if --thresholds is specified)
<BAM-or-CRAM>
the alignment file for which to calculate depth.
Common Options:
-t--threads <threads>
number of BAM decompression threads [default: 0]
-c--chrom <chrom>
chromosome to restrict depth calculation.
-b--by <bed|window>
optional BED file or (integer) window-sizes.
-n--no-per-base
dont output per-base depth. skipping this output will speed execution substantially. prefer
quantized or thresholded values if possible.
-f--fasta <fasta>
fasta file for use with CRAM files [default: ].
Other options:
-F--flag <FLAG>
exclude reads with any of the bits in FLAG set [default: 1796]
-i--include-flag <FLAG>
only include reads with any of the bits in FLAG set. default is unset. [default: 0]
-x--fast-mode
dont look at internal cigar operations or correct mate overlaps (recommended for most use-cases).
-q--quantize <segments>
write quantized output see docs for description.
-Q--mapq <mapq>
mapping quality threshold. reads with a quality less than this value are ignored [default: 0]
-l--min-frag-len <min-frag-len>
minimum insert size. reads with a smaller insert size than this are ignored [default: -1]-u--max-frag-len <max-frag-len>
maximum insert size. reads with a larger insert size than this are ignored. [default: -1]-T--thresholds <thresholds>
for each interval in --by, write number of bases covered by at least threshold bases. Specify
multiple integer values separated by ','.
-m--use-median
output median of each region (in --by) instead of mean.
-R--read-groups <string>
only calculate depth for these comma-separated read groups IDs.
-h--help
show help
mosdepth 0.3.10 December 2024 MOSDEPTH(1)