needle-count - Get expression value depending on minimizers. This function is an alternative to
Contents
Legal
needle-countCopyright: BSD 3-Clause License
Author: Mitra Darvish
SeqAnCopyright: 2006-2023 Knut Reinert, FU-Berlin; released under the 3-clause BSDL.
Inyouracademicworkspleasecite: Needle: a fast and space-efficient prefilter for estimating the
quantification of very large collections of expression experiments; Mitra Darvish, Enrico Seiler, Svenja
Mehringer, René Rahn, and Knut Reinert; Bioinformatics, Volume 38, Issue 17, 1 September 2022, Pages
4100-4108. doi: https://doi.org/10.1093/bioinformatics/btac492
For full copyright and/or warranty information see --copyright.
needle-count 1.0.3 2024-11-07 NEEDLE-COUNT(1)
Name
needle-count - Get expression value depending on minimizers. This function is an alternative to
pseudoaligners like kallisto. It estimates the expression value for all sequences in the genome file
based on the exact minimiser occurrences of the given sequence files. Please run genome beforehand to
create the genome file.
Options
Basicoptions:-h, --help
Prints the help page.
-hh, --advanced-help
Prints the help page including advanced options.
--version
Prints the version information.
--copyright
Prints the copyright/license information.
--export-help (std::string)
Export the help page information. Value must be one of [html, man].
-k, --kmer (unsigned8bitinteger)
Define k-mer size for the minimisers. Default: 20. Default: 20.
-w, --window (unsigned32bitinteger)
Define window size for the minimisers. Default: 60. Default: 0.
--shape (unsigned64bitinteger)
Define a shape for the minimisers by the decimal of a bitvector, where 0 symbolizes a position to
be ignored, 1 a position considered. Default: ungapped. Default: 0.
--seed (unsigned64bitinteger)
Define seed for the minimisers. Default: 0.
-o, --out (std::filesystem::path)
Directory, where output files should be saved. Default: "./".
-t, --threads (unsigned8bitinteger)
Number of threads to use. Default: 1. Default: 1.
--include (std::filesystem::path)
Please provide one sequence file with transcripts.
--genome (std::filesystem::path)
Please provide one *.genome file created with the genome command. Default: "".
-p, --paired
If set, experiments are paired. Default: Not paired.
Positional Arguments
ARGUMENT-1 (List of std::filesystem::path)
Please provide at least one sequence file. Default: [].
Url
https://github.com/seqan/needle
Version
Lastupdate: 2024-11-07
needle-countversion: 1.0.3
SeqAnversion: 3.3.0
