pair_align - Pairwise alignment
Contents
Alignment Configuration
The alignment configuration is a string of four characters, each being either t or f. All combinations
are allowed. The meaning is as follows.
tfff First row initialized with 0s.
ftff First column initialized with 0s.
fftf Search last column for maximum.
ffft Search last row for maximum.
pair_align 1.3.8 [tarball] PAIR_ALIGN(1)
Description
The program allows one to align two sequences using dyamic programming alignment algorithms while
tweaking various parameters.
Name
pair_align - Pairwise alignment
Options
-h, --help
Display the help message.
--version
Display version information.
MainOptions:-s, --seqINPUT_FILE
FASTA file with two sequences. Valid filetypes are: .frn, .fna, .ffn, .fasta, .faa, and .fa.
-a, --alphabetSTRING
Sequence alphabet. One of protein, dna, rna, and text. Default: protein.
-m, --methodSTRING
DP alignment method: Needleman-Wunsch, Gotoh, Smith-Waterman, Longest Common Subsequence One of
nw, gotoh, sw, and lcs. Default: gotoh.
-o, --outfileOUTPUT_FILE
Output filename. Valid filetypes are: .msf, .frn, .fna, .ffn, .fasta, .faa, and .fa. Default:
out.fasta.
ScoringOptions:-g, --gopINTEGER
Gap open penalty. Default: -11.
-e, --gexINTEGER
Gap extension penalty. Default: -1.
-ma, --matrixSTRING
Score matrix.
-ms, --mscINTEGER
Match score. Default: 5.
-mm, --mmscINTEGER
Mismatch penalty. Default: -4.
BandedAlignmentOptions:-lo, --lowINTEGER
Lower diagonal.
-hi, --highINTEGER
Upper diagonal.
DPMatrixConfigurationOptions:-c, --configSTRING
Alignment configuration. One of ffff, ffft, fftf, fftt, ftff, ftft, fttf, fttt, tfff, tfft, tftf,
tftt, ttff, ttft, tttf, and tttt.
Synopsis
pair_align [OPTIONS] -sIN
