-rSTRING
Allows you to specify a read group line to append to the header and applies it to the reads
specified by the -m option. If repeated it automatically adds in tabs between invocations.
-RSTRING
Allows you to specify the read group ID of an existing @RG line and applies it to the reads
specified.
-mMODE If you choose orphan_only then existing RG tags are not overwritten, if you choose overwrite_all,
existing RG tags are overwritten. The default is overwrite_all.
-oSTRING
Write the final output to STRING. The default is to write to stdout.
By default, samtools tries to select a format based on the output filename extension; if output
is to standard output or no format can be deduced, sam is selected.
-u Output uncompressed SAM, BAM or CRAM.
-w Overwrite an existing @RG line, if a new one with the same ID value is provided.
--no-PG Do not add a @PG line to the header of the output file.
-@,--threadsINT
Number of input/output compression threads to use in addition to main thread [0].