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reapr-perfectfrombam - generate perfect mapping plots from a bam file

Description

       Alternative to using perfectmap, for large genomes.

       Takes a BAM, which must have AS:... tags in each line. Makes file of perfect mapping depth, for use with
       the REAPR pipeline. Recommended to use perfectmap instead, unless your genome is large (more than
       ~300MB), since although very fast to run, 'reapr perfectmap' uses a lot of memory.

       A BAM file made by smaltmap is suitable input.

       Reads in pair pointing towards each other, with the given minimum alignment score and mapping quality and
       within the given insert size range are used to generate the coverage across the genome.

       Additionally, regions with repetitive coverage are called, by taking read pairs where at least one read
       of the pair (is mapped and) has mapping quality less than or equal to <repetitive max qual>.

Name

       reapr-perfectfrombam - generate perfect mapping plots from a bam file

Options

-noclean
           Use this to not delete the temporary bam file

See Also

reapr(1)

                                                                                         REAPR-PERFECTFROMBAM(1)

Synopsis

reaprperfectfrombam [options] <in.bam> <outfileprefix> <mininsert> <maxinsert> <repetitive-max-qual>
       <perfect-min-qual> <perfect-min-alignment-score>

See Also