samtools-rmdup - removes duplicate reads (obsolete)
Contents
Description
Thiscommandisobsolete.Usemarkdupinstead.
Remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain
the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR
orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends
mapped to different chromosomes or orphan reads).
Limitations
o Samtools paired-end rmdup does not work for unpaired reads (e.g. orphan reads or ends mapped to
different chromosomes). If this is a concern, please use Picard's MarkDuplicates which correctly
handles these cases, although a little slower.
Name
samtools-rmdup - removes duplicate reads (obsolete)
Options
-s Remove duplicates for single-end reads. By default, the command works for paired-end reads only.
-S Treat paired-end reads and single-end reads.
See Also
samtools(1) Samtools website: <http://www.htslib.org/> samtools-1.21 12 September 2024 samtools-rmdup(1)
Synopsis
samtools rmdup [-sS] input.srt.bamout.bam