SPRAI - single-pass nucleotide sequencing read accuracy improvement
Contents
Description
Sprai is a tool to correct sequencing errors in single-pass reads for de novo assembly. It is originally
designed for correcting sequencing errors in single-molecule DNA sequencing reads, especially in
Continuous Long Reads (CLRs) generated by PacBio RS sequencers. The goal of Sprai is not maximizing the
accuracy of error-corrected reads. Instead, Sprai aims at maximizing the continuity (i.e., N50 contig
length) of assembled contigs after error correction.
Name
SPRAI - single-pass nucleotide sequencing read accuracy improvement
Options
-n Show parameters in ec.spec and exit.
-ec_only
Perform error correction only. With this option, asm.spec does not need to be passed in, as
assembly is not performed.
-nowyyyymmdd_hhmmss
Use an existing result_yyyymmdd_hhmmss directory from a previous run, detect unfinished jobs, and
restart at the appropriate stage.
See Also
bash5tools(1) This program is part of sprai. See the sprai package documentation in /usr/share/doc/sprai, especially the example ec and pbasm spec files in /usr/share/doc/sprai/examples June 2016 SPRAI(1)
Synopsis
ezez_vx1ec.spec {asm.spec | -ec_only} [options]
ezez4qsub_vx1ec.spec {asm.spec | -ec_only} [options]
ezez4makefile_v4.plec.spec [asm.spec] && make -jnproc [ec_only]
