velveth - simple hashing program
Contents
Copyright
Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk)
This is free software; see the source for copying conditions. There is NO warranty; not even for
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.
Compilation settings: CATEGORIES = 2 MAXKMERLENGTH = 31
Usage: ./velveth directory hash_length {[-file_format][-read_type][-separate|-interleaved] filename1
[filename2 ...]} {...} [options]
directory
: directory name for output files
hash_length
: EITHER an odd integer (if even, it will be decremented) <= 31 (if above, will be reduced) : OR:
m,M,s where m and M are odd integers (if not, they will be decremented) with m < M <= 31 (if
above, will be reduced)
and s is a step (even number). Velvet will then hash from k=m to k=M with a step of s
filename
: path to sequence file or - for standard input
Fileformatoptions:-fasta-fastq-raw-fasta.gz -fastq.gz -raw.gz -sam-bam-fmtAuto
(Note: -fmtAuto will detect fasta or fastq, and will try the following programs for decompression
: gunzip, pbunzip2, bunzip2
Filelayoutoptionsforpairedreads(onlyforfastaandfastqformats):-interleaved
: File contains paired reads interleaved in the one file (default)
-separate
: Read 2 separate files for paired reads
Readtypeoptions:-short-shortPaired-short2-shortPaired2-long-longPaired-reference
velveth 1.2.10+dfsg1 July 2018 VELVETH(1)
Description
velveth - simple hashing program Version 1.2.09
Name
velveth - simple hashing program
Options
-strand_specific
: for strand specific transcriptome sequencing data (default: off)
-reuse_Sequences
: reuse Sequences file (or link) already in directory (no need to provide original filenames in
this case (default: off)
-reuse_binary
: reuse binary sequences file (or link) already in directory (no need to provide original
filenames in this case (default: off)
-noHash
: simply prepare Sequences file, do not hash reads or prepare Roadmaps file (default: off)
-create_binary
: create binary CnyUnifiedSeq file (default: off)
Synopsis:
-Shortsingleendreads:
velveth Assem 29 -short-fastq s_1_sequence.txt
-Paired-endshortreads(remembertointerleavepairedreads):
velveth Assem 31 -shortPaired-fasta interleaved.fna
- Paired-end short reads (using separate files for the paired reads)
velveth Assem 31 -shortPaired-fasta-separate left.fa right.fa
-Twochannelsandsomelongreads:
velveth Assem 43 -short-fastq unmapped.fna -longPaired-fasta SangerReads.fasta
-Threechannels:
velveth Assem 35 -shortPaired-fasta pe_lib1.fasta -shortPaired2 pe_lib2.fasta -short3 se_lib1.fa
Output:
directory/Roadmaps directory/Sequences
[Both files are picked up by graph, so please leave them there]
