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indexdb_rna - tool for filtering, mapping and OTU-picking NGS reads (indexdb)

Description

       SortMeRNA  is  a  biological sequence analysis tool for filtering, mapping and OTU-picking NGS reads. The
       core algorithm is based on approximate seeds and allows for fast and  sensitive  analyses  of  nucleotide
       sequences.  The main application of SortMeRNA is filtering rRNA from metatranscriptomic data.  Additional
       applications include OTU-picking and taxonomy assignation available through QIIME v1.9+ (http://qiime.org
       - v1.9.0-rc1).

       SortMeRNA takes as input a file of reads (fasta or fastq  format)  and  one  or  multiple  rRNA  database
       file(s), and sorts apart rRNA and rejected reads into two files specified by the user. Optionally, it can
       provide  high  quality  local  alignments  of  rRNA reads against the rRNA database. SortMeRNA works with
       Illumina, 454, Ion Torrent and PacBio data, and can produce SAM and BLAST-like alignments.

Name

       indexdb_rna - tool for filtering, mapping and OTU-picking NGS reads (indexdb)

Options

MANDATORYOPTIONS--refSTRING,STRING
              FASTA reference file, index file
              Example:
              --ref/path/to/file1.fasta,/path/to/index1
              If passing multiple reference sequence files, separate them by ':'
              Example:
              --ref/path/f1.fasta,/path/index1:/path/f2.fasta,path/index2OPTIONALOPTIONS--fastBOOL
              suggested option for aligning ~99% related species (default: off)

       --sensitiveBOOL
              suggested option for aligning ~75-98% related species (default: on)

       --tmpdirSTRING
              directory where to write temporary files

       -mINT the amount of memory (in Mbytes) for building the index (default: 3072)

       -LINT seed length (default: 18)

       --max_posINT
              maximum number of positions to store for each unique L-mer (default: 10000,  setting  --max_pos  0
              will store all positions)

       -vBOOL
              verbose

       -hBOOL
              help

indexdb_rna 2.0                                    August 2015                                    INDEXDB_RNA(1)

Synopsis

indexdb_rna--ref db.fasta,db.idx [OPTIONS]

See Also