indexdb_rna - tool for filtering, mapping and OTU-picking NGS reads (indexdb)
Contents
Description
SortMeRNA is a biological sequence analysis tool for filtering, mapping and OTU-picking NGS reads. The
core algorithm is based on approximate seeds and allows for fast and sensitive analyses of nucleotide
sequences. The main application of SortMeRNA is filtering rRNA from metatranscriptomic data. Additional
applications include OTU-picking and taxonomy assignation available through QIIME v1.9+ (http://qiime.org
- v1.9.0-rc1).
SortMeRNA takes as input a file of reads (fasta or fastq format) and one or multiple rRNA database
file(s), and sorts apart rRNA and rejected reads into two files specified by the user. Optionally, it can
provide high quality local alignments of rRNA reads against the rRNA database. SortMeRNA works with
Illumina, 454, Ion Torrent and PacBio data, and can produce SAM and BLAST-like alignments.
Name
indexdb_rna - tool for filtering, mapping and OTU-picking NGS reads (indexdb)
Options
MANDATORYOPTIONS--refSTRING,STRING
FASTA reference file, index file
Example:
--ref/path/to/file1.fasta,/path/to/index1
If passing multiple reference sequence files, separate them by ':'
Example:
--ref/path/f1.fasta,/path/index1:/path/f2.fasta,path/index2OPTIONALOPTIONS--fastBOOL
suggested option for aligning ~99% related species (default: off)
--sensitiveBOOL
suggested option for aligning ~75-98% related species (default: on)
--tmpdirSTRING
directory where to write temporary files
-mINT the amount of memory (in Mbytes) for building the index (default: 3072)
-LINT seed length (default: 18)
--max_posINT
maximum number of positions to store for each unique L-mer (default: 10000, setting --max_pos 0
will store all positions)
-vBOOL
verbose
-hBOOL
help
indexdb_rna 2.0 August 2015 INDEXDB_RNA(1)
Synopsis
indexdb_rna--ref db.fasta,db.idx [OPTIONS]
