usage: macs2 predictd [-h] -i IFILE [IFILE ...]
[-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}]
[-g GSIZE] [-s TSIZE] [--bw BW] [--d-min D_MIN] [-m MFOLD MFOLD] [--outdir OUTDIR] [--rfile RFILE]
[--buffer-size BUFFER_SIZE] [--verbose VERBOSE]
options:-h, --help
show this help message and exit
-i IFILE [IFILE ...], --ifile IFILE [IFILE ...]
ChIP-seq alignment file. If multiple files are given as '-t A B C', then they will all be read and
combined. Note that pair-end data is not supposed to work with this command. REQUIRED.
-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}, --format
{AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}
Format of tag file, "AUTO", "BED" or "ELAND" or "ELANDMULTI" or "ELANDEXPORT" or "SAM" or "BAM" or
"BOWTIE" or "BAMPE" or "BEDPE". The default AUTO option will let MACS decide which format the file
is. Please check the definition in README file if you choose
ELAND/ELANDMULTI/ELANDEXPORT/SAM/BAM/BOWTIE. DEFAULT: "AUTO"
-g GSIZE, --gsize GSIZE
Effective genome size. It can be 1.0e+9 or 1000000000, or shortcuts:'hs' for human (2.7e9), 'mm'
for mouse (1.87e9), 'ce' for C. elegans (9e7) and 'dm' for fruitfly (1.2e8), Default:hs
-s TSIZE, --tsize TSIZE
Tag size. This will override the auto detected tag size. DEFAULT: Not set
--bw BW
Band width for picking regions to compute fragment size. This value is only used while building
the shifting model. DEFAULT: 300
--d-min D_MIN
Minimum fragment size in basepair. Any predicted fragment size less than this will be excluded.
DEFAULT: 20
-m MFOLD MFOLD, --mfold MFOLD MFOLD
Select the regions within MFOLD range of highconfidence enrichment ratio against background to
build model. Fold-enrichment in regions must be lower than upper limit, and higher than the lower
limit. Use as "-m 10 30". DEFAULT:5 50
--outdir OUTDIR
If specified all output files will be written to that directory. Default: the current working
directory
--rfile RFILE
PREFIX of filename of R script for drawing X-correlation figure. DEFAULT:'predictd' and R file
will be predicted_model.R
--buffer-size BUFFER_SIZE
Buffer size for incrementally increasing internal array size to store reads alignment information.
In most cases, you don't have to change this parameter. However, if there are large number of
chromosomes/contigs/scaffolds in your alignment, it's recommended to specify a smaller buffer size
in order to decrease memory usage (but it will take longer time to read alignment files). Minimum
memory requested for reading an alignment file is about # of CHROMOSOME * BUFFER_SIZE * 8 Bytes.
DEFAULT: 100000
--verbose VERBOSE
Set verbose level of runtime message. 0: only show critical message, 1: show additional warning
message, 2: show process information, 3: show debug messages. DEFAULT:2
macs2 predictd 2.2.7.1 April 2022 MACS2_PREDICTD(1)