prima - Selects primers for PCR and DNA amplification.

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           Wrote the script used to autogenerate this manual page.

       Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to
       the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).

       This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be
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EMBOSS 6.4.0                                       05/11/2012                                          PRIMA(1e)

prima is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is
       part of the "Nucleic:Primers" command group(s).

       prima - Selects primers for PCR and DNA amplification.

Inputsection-sequencesequenceRequiredsectionTargetoptions-targetrangetoggle
           Default value: N

       -targetstartinteger
           Default value: $(sequence.begin)

       -targetendinteger
           Default value: $(sequence.length)

       -mintmprimerfloat
           Default value: 53

       -maxtmprimerfloat
           Default value: 58

   AdditionalsectionProductoptions-minpleninteger
           Default value: 100

       -maxpleninteger
           Default value: 300

       -minpgccontfloat
           Default value: .40

       -maxpgccontfloat
           Default value: .55

   Primeroptions-primertoggle
           Specify primer properties Default value: Y

       -minprimerleninteger
           Default value: 18

       -maxprimerleninteger
           Default value: 22

       -minpmgccontfloat
           Default value: .40

       -maxpmgccontfloat
           Default value: .55

   Advancedsection-overlapinteger
           Default value: 50

       -saltconcfloat
           Default value: 50

       -dnaconcfloat
           Default value: 50

       -listboolean
           Default value: N

   Outputsection-outfileoutfile

       prima is fully documented via the tfm(1) system.

prima-sequencesequence-targetrangetoggle-targetstartinteger-targetendinteger-mintmprimerfloat-maxtmprimerfloat-minpleninteger-maxpleninteger-minpgccontfloat-maxpgccontfloat
             [-primertoggle] -minprimerleninteger-maxprimerleninteger-minpmgccontfloat-maxpmgccontfloat-overlapinteger-saltconcfloat-dnaconcfloat-listboolean-outfileoutfileprima-help