Inputsection-sequenceseqallAdditionalsection-tablelist-minsizeinteger
Default value: 30
-maxsizeinteger
Default value: 1000000
-findlist
This is a small menu of possible output options. The first four options are to select either the
protein translation or the original nucleic acid sequence of the open reading frame. There are two
possible definitions of an open reading frame: it can either be a region that is free of STOP codons
or a region that begins with a START codon and ends with a STOP codon. The last three options are
probably only of interest to people who wish to investigate the statistical properties of the regions
around potential START or STOP codons. The last option assumes that ORF lengths are calculated
between two STOP codons.
Advancedsection-methionineboolean
START codons at the beginning of protein products will usually code for Methionine, despite what the
codon will code for when it is internal to a protein. This qualifier sets all such START codons to
code for Methionine by default. Default value: Y
-circularboolean
Default value: N
-reverseboolean
Set this to be false if you do not wish to find ORFs in the reverse complement of the sequence.
Default value: Y
-flankinginteger
If you have chosen one of the options of the type of sequence to find that gives the flanking
sequence around a STOP or START codon, this allows you to set the number of nucleotides either side
of that codon to output. If the region of flanking nucleotides crosses the start or end of the
sequence, no output is given for this codon. Default value: 100
Outputsection-outseqseqoutall