usage: macs2 randsample [-h] -i IFILE [IFILE ...] (-p PERCENTAGE | -n NUMBER)
[--seed SEED] [-o OUTPUTFILE] [--outdir OUTDIR]
[-s TSIZE] [-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}] [--buffer-size
BUFFER_SIZE] [--verbose VERBOSE]
options:-h, --help
show this help message and exit
-i IFILE [IFILE ...], --ifile IFILE [IFILE ...]
Alignment file. If multiple files are given as '-t A B C', then they will all be read and
combined. Note that pair-end data is not supposed to work with this command. REQUIRED.
-p PERCENTAGE, --percentage PERCENTAGE
Percentage of tags you want to keep. Input 80.0 for 80%. This option can't be used at the same
time with -n/--num. REQUIRED
-n NUMBER, --number NUMBER
Number of tags you want to keep. Input 8000000 or 8e+6 for 8 million. This option can't be used at
the same time with -p/--percent. Note that the number of tags in output is approximate as the
number specified here. REQUIRED
--seed SEED
Set the random seed while down sampling data. Must be a non-negative integer in order to be
effective. DEFAULT: not set
-o OUTPUTFILE, --ofile OUTPUTFILE
Output BED file name. If not specified, will write to standard output. Note, if the input format
is BAMPE or BEDPE, the output will be in BEDPE format. DEFAULT: stdout
--outdir OUTDIR
If specified all output files will be written to that directory. Default: the current working
directory
-s TSIZE, --tsize TSIZE
Tag size. This will override the auto detected tag size. DEFAULT: Not set
-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}, --format
{AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}
Format of tag file, "AUTO", "BED" or "ELAND" or "ELANDMULTI" or "ELANDEXPORT" or "SAM" or "BAM" or
"BOWTIE" or "BAMPE" or "BEDPE". The default AUTO option will macs2 randsample decide which format
the file is. Please check the definition in README file if you choose
ELAND/ELANDMULTI/ELANDEXPORT/SAM/BAM/BOWTIE or BAMPE/BEDPE. DEFAULT: "AUTO"
--buffer-size BUFFER_SIZE
Buffer size for incrementally increasing internal array size to store reads alignment information.
In most cases, you don't have to change this parameter. However, if there are large number of
chromosomes/contigs/scaffolds in your alignment, it's recommended to specify a smaller buffer size
in order to decrease memory usage (but it will take longer time to read alignment files). Minimum
memory requested for reading an alignment file is about # of CHROMOSOME * BUFFER_SIZE * 8 Bytes.
DEFAULT: 100000
--verbose VERBOSE
Set verbose level. 0: only show critical message, 1: show additional warning message, 2: show
process information, 3: show debug messages. If you want to know where are the duplicate reads,
use 3. DEFAULT:2
macs2 randsample 2.2.7.1 April 2022 MACS2_RANDSAMPLE(1)