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seqtk - sampling, trimming, fastq2fasta, subsequence, reverse complement

Commands

       Command: seq       common transformation of FASTA/Q

       comp   get the nucleotide composition of FASTA/Q

       sample subsample sequences

       subseq extract subsequences from FASTA/Q

       trimfq trim FASTQ using the Phred algorithm

       hety   regional heterozygosity

       mutfa  point mutate FASTA at specified positions

       mergefa
              merge two FASTA/Q files

       randbase
              choose a random base from hets

       cutN   cut sequence at long N

       listhet
              extract the position of each het

seqtk 1.0                                         February 2014                                         SEQTK(1)

Description

       Currently,  seqtk  supports  quality  based trimming with the phred algorithm, converting fastq to fasta,
       reverse complementing sequences, extracting or masking subsequences in regions given in a  BED/name  list
       file,  and  more.   It  contains  a  subsampling  module  to  sample exactly n sequences or a fraction of
       sequences.

       Seqtk supports both fasta and fastq input files, which can be optionally gzip compressed.

Name

       seqtk - sampling, trimming, fastq2fasta, subsequence, reverse complement

Synopsis

seqtk<command><arguments>

See Also