seqtk - sampling, trimming, fastq2fasta, subsequence, reverse complement
Contents
Commands
Command: seq common transformation of FASTA/Q
comp get the nucleotide composition of FASTA/Q
sample subsample sequences
subseq extract subsequences from FASTA/Q
trimfq trim FASTQ using the Phred algorithm
hety regional heterozygosity
mutfa point mutate FASTA at specified positions
mergefa
merge two FASTA/Q files
randbase
choose a random base from hets
cutN cut sequence at long N
listhet
extract the position of each het
seqtk 1.0 February 2014 SEQTK(1)
Description
Currently, seqtk supports quality based trimming with the phred algorithm, converting fastq to fasta,
reverse complementing sequences, extracting or masking subsequences in regions given in a BED/name list
file, and more. It contains a subsampling module to sample exactly n sequences or a fraction of
sequences.
Seqtk supports both fasta and fastq input files, which can be optionally gzip compressed.
Name
seqtk - sampling, trimming, fastq2fasta, subsequence, reverse complement
Synopsis
seqtk<command><arguments>
